Optimizing semen cryopreservation in Calomys laucha: a step forward in rodent reproductive research.
Cryo Letters
; 45(2): 134-138, 2024.
Article
em En
| MEDLINE
| ID: mdl-38557992
ABSTRACT
BACKGROUND:
Examining semen cryopreservation in Calomys laucha offers valuable insights for reproductive research and species conservation.OBJECTIVE:
To determine the most effective sugar for the cryopreservation of C. laucha semen. MATERIALS ANDMETHODS:
Using 36 epididymides from C. laucha, semen samples were diluted in a 3% skimmed milk medium supplemented with one of four sugars (glucose, fructose, lactose, or sucrose) at a concentration of 0.3 M. These mixtures underwent a conditioning phase at 37 degree C for 10 min, cooled to -80 degree C for another 10 min, and were subsequently stored in liquid nitrogen.RESULTS:
Upon thawing, samples treated with lactose and glucose solutions show superior sperm motility, achieving 8.2% and 10.0% respectively, in contrast to the fructose (2.0%) and sucrose (4.1%) mixtures. Furthermore, samples preserved in glucose registered the highest sperm penetration rates, reaching 44.9%.CONCLUSION:
Our findings suggest that a cryopreservation medium containing 0.3 M glucose can contribute to the safeguarding C. laucha rodent semen. https//doi.org/10.54680/fr24210110612.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sêmen
/
Preservação do Sêmen
Limite:
Animals
Idioma:
En
Revista:
Cryo Letters
Ano de publicação:
2024
Tipo de documento:
Article