Rapid antibiotic susceptibility testing (RAST) of blood cultures in enterobacteria with inducible chromosomal AmpC-type ß-lactamase.
Enferm Infecc Microbiol Clin (Engl Ed)
; 42(4): 205-207, 2024 Apr.
Article
em En
| MEDLINE
| ID: mdl-38575276
ABSTRACT
INTRODUCTION:
Early and adequate treatment of bloodstream infections decreases patient morbidity and mortality. The objective is to develop a preliminary method for rapid antibiotic susceptibility testing (RAST) in enterobacteria with inducible chromosomal AmpC.METHODS:
RAST was performed directly on spiked blood cultures of 49 enterobacteria with inducible chromosomal AmpC. Results were read at 4, 6 and 8h of incubation. Commercial broth microdilution was considered the reference method. Disks of 10 antibiotics were evaluated.RESULTS:
The proportion of readable tests at 4h was 85%. All RAST could be read at 6 and 8h. For most antibiotics, the S or R result at 4, 6 and 8h was greater than 80% after tentative breakpoints were established and Area of Technical Uncertainty was defined.CONCLUSIONS:
This preliminary method seems to be of practical use, although it should be extended to adjust the breakpoints and differentiate them by species.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Enterobacteriaceae
/
Hemocultura
Limite:
Humans
Idioma:
En
Revista:
Enferm Infecc Microbiol Clin (Engl Ed)
Ano de publicação:
2024
Tipo de documento:
Article