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ptx3a+ fibroblast/epicardial cells provide a transient macrophage niche to promote heart regeneration.
Sun, Jisheng; Peterson, Elizabeth A; Chen, Xin; Wang, Jinhu.
Afiliação
  • Sun J; Cardiology Division, School of Medicine, Emory University, Atlanta, GA 30322, USA.
  • Peterson EA; Cardiology Division, School of Medicine, Emory University, Atlanta, GA 30322, USA.
  • Chen X; Cardiology Division, School of Medicine, Emory University, Atlanta, GA 30322, USA.
  • Wang J; Cardiology Division, School of Medicine, Emory University, Atlanta, GA 30322, USA. Electronic address: jinhu.wang@emory.edu.
Cell Rep ; 43(4): 114092, 2024 Apr 23.
Article em En | MEDLINE | ID: mdl-38607913
ABSTRACT
Macrophages conduct critical roles in heart repair, but the niche required to nurture and anchor them is poorly studied. Here, we investigated the macrophage niche in the regenerating heart. We analyzed cell-cell interactions through published single-cell RNA sequencing datasets and identified a strong interaction between fibroblast/epicardial (Fb/Epi) cells and macrophages. We further visualized the association of macrophages with Fb/Epi cells and the blockage of macrophage response without Fb/Epi cells in the regenerating zebrafish heart. Moreover, we found that ptx3a+ epicardial cells associate with reparative macrophages, and their depletion resulted in fewer reparative macrophages. Further, we identified csf1a expression in ptx3a+ cells and determined that pharmacological inhibition of the csf1a pathway or csf1a knockout blocked the reparative macrophage response. Moreover, we found that genetic overexpression of csf1a enhanced the reparative macrophage response with or without heart injury. Altogether, our studies illuminate a cardiac Fb/Epi niche, which mediates a beneficial macrophage response after heart injury.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regeneração / Peixe-Zebra / Fibroblastos / Coração / Macrófagos Limite: Animals Idioma: En Revista: Cell Rep Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regeneração / Peixe-Zebra / Fibroblastos / Coração / Macrófagos Limite: Animals Idioma: En Revista: Cell Rep Ano de publicação: 2024 Tipo de documento: Article