Your browser doesn't support javascript.
loading
Investigations of Limeum Indicum Plant for Diabetes Mellitus and Alzheimer's Disease Dual Therapy: Phytochemical, GC-MS Chemical Profiling, Enzyme Inhibition, Molecular Docking and In-Vivo Studies.
Shamim, Tahira; Asif, Hafiz Muhammad; Abida Ejaz, Syeda; Hussain, Zahid; Wani, Tanveer A; Sumreen, Laila; Abdullah, Muhammad; Ahmed, Zubair; Iqbal, Jamshed; Kim, Song Ja; Shah, Muhammad Kamal.
Afiliação
  • Shamim T; University College of Conventional Medicine, Faculty of Medicine & Allied Health Sciences, The Islamia University of Bahawalpur, 63100, Bahawalpur, Pakistan.
  • Asif HM; University College of Conventional Medicine, Faculty of Medicine & Allied Health Sciences, The Islamia University of Bahawalpur, 63100, Bahawalpur, Pakistan.
  • Abida Ejaz S; Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The Islamia University of Bahawalpur, 63100, Bahawalpur, Pakistan.
  • Hussain Z; Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, 22060, Abbottabad, Pakistan.
  • Wani TA; Center for Advance Drug Research, COMSATS University Islamabad, Abbottabad Campus, 22060, Abbottabad, Pakistan.
  • Sumreen L; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O.Box 2452, 11451, Riyadh, Saudi Arabia.
  • Abdullah M; University College of Conventional Medicine, Faculty of Medicine & Allied Health Sciences, The Islamia University of Bahawalpur, 63100, Bahawalpur, Pakistan.
  • Ahmed Z; Cholistan Institute of Desert Studies, The Islamia University of Bahawalpur, 63100, Bahawalpur, Pakistan.
  • Iqbal J; Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, 22060, Abbottabad, Pakistan.
  • Kim SJ; Department of Chemistry, COMSATS University Islamabad, Abbottabad Campus, 22060, Abbottabad, Pakistan.
  • Shah MK; Center for Advance Drug Research, COMSATS University Islamabad, Abbottabad Campus, 22060, Abbottabad, Pakistan.
Chem Biodivers ; 21(6): e202301858, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38608202
ABSTRACT
Limeum indicum has been widely utilized in traditional medicine but no experimental work has been done on this herb. The primary objective of this study was to conduct a phytochemical analysis and assess the multifunctional capabilities of aforementioned plant in dual therapy for Alzheimer's disease (AD) and Type 2 diabetes (T2D). The phytochemical screening of ethanol, methanol extract, and their derived fractions of Limeum indicum was conducted using GC-MS, HPLC, UV-analysis and FTIR. The antioxidant capacity was evaluated by DPPH method. The inhibitory potential of the extracts/fractions against α-, ß-glucosidase acetylcholinesterase (AChE), butyrylcholinesterase (BChE) and monoaminine oxidases (MAO-A & B) was evaluated. Results revealed that acetonitrile fraction has highest inhibitory potential against α-glucosidase (IC50=68.47±0.05 µg/mL), methanol extract against ß-glucosidase (IC50=91.12±0.07 µg/mL), ethyl acetate fraction against AChE (IC50=59.0±0.02 µg/mL), ethanol extract against BChE (28.41±0.01 µg/mL), n-hexane fraction against MAO-A (IC50=150.5±0.31 µg/mL) and methanol extract for MAO-B (IC50=75.95±0.13 µg/mL). The docking analysis of extracts\fractions suggested the best binding scores within the active pocket of the respective enzymes. During the in-vivo investigation, ethanol extract produced hypoglycemic effect (134.52±2.79 and 119.38±1.40 mg/dl) after 21 days treatment at dose level of 250 and 500 mg/Kg. Histopathological findings further supported the in-vivo studies.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Acetilcolinesterase / Butirilcolinesterase / Extratos Vegetais / Doença de Alzheimer / Simulação de Acoplamento Molecular / Compostos Fitoquímicos / Hipoglicemiantes / Cromatografia Gasosa-Espectrometria de Massas / Monoaminoxidase Limite: Animals / Humans / Male Idioma: En Revista: Chem Biodivers Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Acetilcolinesterase / Butirilcolinesterase / Extratos Vegetais / Doença de Alzheimer / Simulação de Acoplamento Molecular / Compostos Fitoquímicos / Hipoglicemiantes / Cromatografia Gasosa-Espectrometria de Massas / Monoaminoxidase Limite: Animals / Humans / Male Idioma: En Revista: Chem Biodivers Ano de publicação: 2024 Tipo de documento: Article