Novel approach for detecting classical swine fever virus from swabs of wild boar cut tails using nested real-time PCR.
J Vet Med Sci
; 86(6): 656-659, 2024 Jun 01.
Article
em En
| MEDLINE
| ID: mdl-38658334
ABSTRACT
We devised a method to detect the classical swine fever virus (CSFV) in tail-wiped swabs from wild boars. The CSFV gene in swabs was detected with high sensitivity using nested real-time polymerase chain reaction (PCR), which is a combination of reverse transcription-PCR (RT-PCR) and real-time PCR. We compared CSFV gene detection from boar tissue using the conventional and our tail-wiped swab method. The tail-wiped swab method showed sensitivity and specificity of 100% (26/26) and 98.8% (172/174), respectively compared to the conventional method. Thus, the swab-based CSFV detection method was considered to have detection sensitivity comparable to that of conventional methods. Additionally, we conducted surveillance for CSFV in wild boars on Awaji Island. CSFV was detected in 10.7% (45/420) of samples.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Sensibilidade e Especificidade
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Sus scrofa
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Reação em Cadeia da Polimerase em Tempo Real
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Peste Suína Clássica
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Vírus da Febre Suína Clássica
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
J Vet Med Sci
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J. vet. med. sci. (Online)
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Journal of veterinary medical science (Online)
Ano de publicação:
2024
Tipo de documento:
Article