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Metabolic coupling between soil aerobic methanotrophs and denitrifiers in rice paddy fields.
Chen, Kang-Hua; Feng, Jiao; Bodelier, Paul L E; Yang, Ziming; Huang, Qiaoyun; Delgado-Baquerizo, Manuel; Cai, Peng; Tan, Wenfeng; Liu, Yu-Rong.
Afiliação
  • Chen KH; National Key Laboratory of Agricultural Microbiology and College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China.
  • Feng J; State Environmental Protection Key Laboratory of Soil Health and Green Remediation and Hubei Key Laboratory of Soil Environment and Pollution Remediation, Huazhong Agricultural University, Wuhan, 430070, China.
  • Bodelier PLE; National Key Laboratory of Agricultural Microbiology and College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China. fengjiao@mail.hzau.edu.cn.
  • Yang Z; State Environmental Protection Key Laboratory of Soil Health and Green Remediation and Hubei Key Laboratory of Soil Environment and Pollution Remediation, Huazhong Agricultural University, Wuhan, 430070, China. fengjiao@mail.hzau.edu.cn.
  • Huang Q; Department of Microbial Ecology, Netherlands Institute of Ecology (NIOO-KNAW), PO Box 50, 6700 AB, Wageningen, The Netherlands.
  • Delgado-Baquerizo M; Department of Chemistry, Oakland University, Rochester, MI, 48309, USA.
  • Cai P; National Key Laboratory of Agricultural Microbiology and College of Resources and Environment, Huazhong Agricultural University, Wuhan, 430070, China.
  • Tan W; State Environmental Protection Key Laboratory of Soil Health and Green Remediation and Hubei Key Laboratory of Soil Environment and Pollution Remediation, Huazhong Agricultural University, Wuhan, 430070, China.
  • Liu YR; Laboratorio de Biodiversidad y Funcionamiento Ecosistémico, Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS), CSIC, Sevilla, 41012, Spain.
Nat Commun ; 15(1): 3471, 2024 Apr 24.
Article em En | MEDLINE | ID: mdl-38658559
ABSTRACT
Paddy fields are hotspots of microbial denitrification, which is typically linked to the oxidation of electron donors such as methane (CH4) under anoxic and hypoxic conditions. While several anaerobic methanotrophs can facilitate denitrification intracellularly, whether and how aerobic CH4 oxidation couples with denitrification in hypoxic paddy fields remains virtually unknown. Here we combine a ~3300 km field study across main rice-producing areas of China and 13CH4-DNA-stable isotope probing (SIP) experiments to investigate the role of soil aerobic CH4 oxidation in supporting denitrification. Our results reveal positive relationships between CH4 oxidation and denitrification activities and genes across various climatic regions. Microcosm experiments confirm that CH4 and methanotroph addition promote gene expression involved in denitrification and increase nitrous oxide emissions. Moreover, 13CH4-DNA-SIP analyses identify over 70 phylotypes harboring genes associated with denitrification and assimilating 13C, which are mostly belonged to Rubrivivax, Magnetospirillum, and Bradyrhizobium. Combined analyses of 13C-metagenome-assembled genomes and 13C-metabolomics highlight the importance of intermediates such as acetate, propionate and lactate, released during aerobic CH4 oxidation, for the coupling of CH4 oxidation with denitrification. Our work identifies key microbial taxa and pathways driving coupled aerobic CH4 oxidation and denitrification, with important implications for nitrogen management and greenhouse gas regulation in agroecosystems.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxirredução / Oryza / Solo / Microbiologia do Solo / Desnitrificação / Metano País/Região como assunto: Asia Idioma: En Revista: Nat Commun Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Oxirredução / Oryza / Solo / Microbiologia do Solo / Desnitrificação / Metano País/Região como assunto: Asia Idioma: En Revista: Nat Commun Ano de publicação: 2024 Tipo de documento: Article