Rapid autopsies to enhance metastatic research: the UPTIDER post-mortem tissue donation program.
NPJ Breast Cancer
; 10(1): 31, 2024 Apr 24.
Article
em En
| MEDLINE
| ID: mdl-38658604
ABSTRACT
Research on metastatic cancer has been hampered by limited sample availability. Here we present the breast cancer post-mortem tissue donation program UPTIDER and show how it enabled sampling of a median of 31 (range 5-90) metastases and 5-8 liquids per patient from its first 20 patients. In a dedicated experiment, we show the mild impact of increasing time after death on RNA quality, transcriptional profiles and immunohistochemical staining in tumor tissue samples. We show that this impact can be counteracted by organ cooling. We successfully generated ex vivo models from tissue and liquid biopsies from distinct histological subtypes of breast cancer. We anticipate these and future findings of UPTIDER to elucidate mechanisms of disease progression and treatment resistance and to provide tools for the exploration of precision medicine strategies in the metastatic setting.
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01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
NPJ Breast Cancer
Ano de publicação:
2024
Tipo de documento:
Article