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Emx2 underlies the development and evolution of marsupial gliding membranes.
Moreno, Jorge A; Dudchenko, Olga; Feigin, Charles Y; Mereby, Sarah A; Chen, Zhuoxin; Ramos, Raul; Almet, Axel A; Sen, Harsha; Brack, Benjamin J; Johnson, Matthew R; Li, Sha; Wang, Wei; Gaska, Jenna M; Ploss, Alexander; Weisz, David; Omer, Arina D; Yao, Weijie; Colaric, Zane; Kaur, Parwinder; Leger, Judy St; Nie, Qing; Mena, Alexandria; Flanagan, Joseph P; Keller, Greta; Sanger, Thomas; Ostrow, Bruce; Plikus, Maksim V; Kvon, Evgeny Z; Aiden, Erez Lieberman; Mallarino, Ricardo.
Afiliação
  • Moreno JA; Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
  • Dudchenko O; Stowers Institute for Medical Research, Kansas City, MO, USA.
  • Feigin CY; The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
  • Mereby SA; The Center for Theoretical Biological Physics, Rice University, Houston, TX, USA.
  • Chen Z; Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
  • Ramos R; School of BioSciences, The University of Melbourne, Parkville, Victoria, Australia.
  • Almet AA; Department of Environment and Genetics, La Trobe University, Bundoora, Victoria, Australia.
  • Sen H; Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
  • Brack BJ; Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA, USA.
  • Johnson MR; Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA, USA.
  • Li S; Department of Mathematics, University of California, Irvine, Irvine, CA, USA.
  • Wang W; NSF-Simons Center for Multiscale Cell Fate Research, University of California, Irvine, Irvine, CA, USA.
  • Gaska JM; Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
  • Ploss A; Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
  • Weisz D; Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
  • Omer AD; Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
  • Yao W; Lewis Sigler Center for Integrative Genomics, Princeton University, Princeton, NJ, USA.
  • Colaric Z; Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
  • Kaur P; Department of Molecular Biology, Princeton University, Princeton, NJ, USA.
  • Leger JS; The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
  • Nie Q; The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
  • Mena A; The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
  • Flanagan JP; The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX, USA.
  • Keller G; The University of Western Australia, Crawley, Western Australia, Australia.
  • Sanger T; Cornell University College of Veterinary Medicine, Ithaca, NY, USA.
  • Ostrow B; Department of Developmental and Cell Biology, University of California, Irvine, Irvine, CA, USA.
  • Plikus MV; Department of Mathematics, University of California, Irvine, Irvine, CA, USA.
  • Kvon EZ; NSF-Simons Center for Multiscale Cell Fate Research, University of California, Irvine, Irvine, CA, USA.
  • Aiden EL; Center for Complex Biological Systems, University of California, Irvine, Irvine, CA, USA.
  • Mallarino R; SeaWorld San Diego, San Diego, CA, USA.
Nature ; 629(8010): 127-135, 2024 May.
Article em En | MEDLINE | ID: mdl-38658750
ABSTRACT
Phenotypic variation among species is a product of evolutionary changes to developmental programs1,2. However, how these changes generate novel morphological traits remains largely unclear. Here we studied the genomic and developmental basis of the mammalian gliding membrane, or patagium-an adaptative trait that has repeatedly evolved in different lineages, including in closely related marsupial species. Through comparative genomic analysis of 15 marsupial genomes, both from gliding and non-gliding species, we find that the Emx2 locus experienced lineage-specific patterns of accelerated cis-regulatory evolution in gliding species. By combining epigenomics, transcriptomics and in-pouch marsupial transgenics, we show that Emx2 is a critical upstream regulator of patagium development. Moreover, we identify different cis-regulatory elements that may be responsible for driving increased Emx2 expression levels in gliding species. Lastly, using mouse functional experiments, we find evidence that Emx2 expression patterns in gliders may have been modified from a pre-existing program found in all mammals. Together, our results suggest that patagia repeatedly originated through a process of convergent genomic evolution, whereby regulation of Emx2 was altered by distinct cis-regulatory elements in independently evolved species. Thus, different regulatory elements targeting the same key developmental gene may constitute an effective strategy by which natural selection has harnessed regulatory evolution in marsupial genomes to generate phenotypic novelty.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Proteínas de Homeodomínio / Evolução Molecular / Locomoção / Marsupiais Limite: Animals / Female / Humans / Male Idioma: En Revista: Nature Ano de publicação: 2024 Tipo de documento: Article
Texto completo
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fatores de Transcrição / Proteínas de Homeodomínio / Evolução Molecular / Locomoção / Marsupiais Limite: Animals / Female / Humans / Male Idioma: En Revista: Nature Ano de publicação: 2024 Tipo de documento: Article