Relevance of amphiphilicity and helicity on the antibacterial action of a histatin 5-derived peptide.
J Pept Sci
; 30(9): e3609, 2024 Sep.
Article
em En
| MEDLINE
| ID: mdl-38676397
ABSTRACT
Peptide dhvar4, derived from the active domain of our salivary peptide histatin 5, bears a Phe residue in the middle of its hydrophilic face when folded into an α-helix. We then synthesized an analog with this Phe replaced by Lys and two analogs preserving Phe but bearing two and three α-aminoisobutyric acid (Aib) residues to stabilize the helical structure. The aim of this design was to verify which of the two features is more favorable to the biological activity. We performed a conformational study by means of circular dichroism and nuclear magnetic resonance, made antibacterial tests, and assessed the stability of the peptides in human serum. We observed that amphiphilicity is more important than helix stability, provided a peptide can adopt a helical conformation in a membrane-mimetic environment.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testes de Sensibilidade Microbiana
/
Histatinas
/
Antibacterianos
Limite:
Humans
Idioma:
En
Revista:
J Pept Sci
Ano de publicação:
2024
Tipo de documento:
Article