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Myrosinase isogenes in wasabi (Wasabia japonica Matsum) and their putative roles in glucosinolate metabolism.
Truong, To Quyen; Park, Yun Ji; Jeon, Je-Seung; Choi, Jaeyoung; Koo, Song Yi; Choi, Yeong Bin; Huynh, Phuong Kim; Moon, Jinyoung; Kim, Sang Min.
Afiliação
  • Truong TQ; Division of Bio-Medical Science & Technology, Korea Institute of Science and Technology (KIST), University of Science and Technology, Seoul, 02792, Republic of Korea.
  • Park YJ; Smart Farm Research Center, KIST Gangneung Institute of Natural Products, Gangneung, 25451, Republic of Korea.
  • Jeon JS; Smart Farm Research Center, KIST Gangneung Institute of Natural Products, Gangneung, 25451, Republic of Korea.
  • Choi J; Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science, RDA, Eumseong, 27709, Republic of Korea.
  • Koo SY; Department of Oriental Medicine Biotechnology, College of Life Sciences, Kyung Hee University, Yongin, 17104, Republic of Korea.
  • Choi YB; Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products, Gangneung, 25451, Republic of Korea.
  • Huynh PK; Smart Farm Research Center, KIST Gangneung Institute of Natural Products, Gangneung, 25451, Republic of Korea.
  • Moon J; Division of Bio-Medical Science & Technology, Korea Institute of Science and Technology (KIST), University of Science and Technology, Seoul, 02792, Republic of Korea.
  • Kim SM; Smart Farm Research Center, KIST Gangneung Institute of Natural Products, Gangneung, 25451, Republic of Korea.
BMC Plant Biol ; 24(1): 353, 2024 May 01.
Article em En | MEDLINE | ID: mdl-38693493
ABSTRACT

BACKGROUND:

Wasabi, a Brassicaceae member, is well-known for its unique pungent and hot flavor which is produced from glucosinolate (GSL) degradation. Myrosinase (MYR) is a principle enzyme catalyzing the primary conversion of GSLs to GSL hydrolysis products (GHPs) which is responsible for plant defense system and food quality. Due to the limited information in relation to MYRs present in wasabi (Wasabia japonica M.), this study aimed to identify the MYR isogenes in W. japonica and analyze their roles in relation to GSL metabolism.

RESULTS:

In results, WjMYRI-1 was abundantly expressed in all organs, whereas WjMYRI-2 showed only trace expression levels. WjMYRII was highly expressed in the aboveground tissues. Interestingly, WjMYRII expression was significantly upregulated by certain abiotic factors, such as methyl jasmonate (more than 40-fold in petioles and 15-fold in leaves) and salt (tenfold in leaves). Young leaves and roots contained 97.89 and 91.17 µmol‧g-1 of GSL, whereas less GSL was produced in mature leaves and petioles (38.36 and 44.79 µmol‧g-1, respectively). Similar pattern was observed in the accumulation of GHPs in various plant organs. Notably, despite the non-significant changes in GSL production, abiotic factors treated samples enhanced significantly GHP content. Pearson's correlation analysis revealed that WjMYRI-1 expression significantly correlated with GSL accumulation and GHP formation, suggesting the primary role of WjMYRI-1-encoding putative protein in GSL degradation. In contrast, WjMYRII expression level showed no correlation with GSL or GHP content, suggesting another physiological role of WjMYRII in stress-induced response.

CONCLUSIONS:

In conclusions, three potential isogenes (WjMYRI-1, WjMYRI-2, and WjMYRII) encoding for different MYR isoforms in W. japonica were identified. Our results provided new insights related to MYR and GSL metabolism which are important for the implications of wasabi in agriculture, food and pharmaceutical industry. Particularly, WjMYRI-1 may be primarily responsible for GSL degradation, whereas WjMYRII (clade II) may be involved in other regulatory pathways induced by abiotic factors.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glucosinolatos / Glicosídeo Hidrolases / Acetatos Idioma: En Revista: BMC Plant Biol Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glucosinolatos / Glicosídeo Hidrolases / Acetatos Idioma: En Revista: BMC Plant Biol Ano de publicação: 2024 Tipo de documento: Article