Knockdown of vitamin D receptor affects early stages of pectoral fin development in zebrafish.
Anat Histol Embryol
; 53(3): e13044, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38695121
ABSTRACT
The vitamin D receptor (VDR) signalling has been implicated in vertebrate limb or fin formation. However, the involvement of VDR signalling in the early stages of limb/fin development remains to be elucidated. In this study, the role of VDR signalling in pectoral fin development was investigated in zebrafish embryos. Knockdown of vdr induced the severe impairment of pectoral fin development. The zebrafish larvae lacking vdr exhibited reduced pectoral fins with no skeletal elements. In situ hybridization revealed depletion of vdr downregulated fibroblast growth factor 24 (fgf24), a marker of early pectoral fin bud mesenchyme, in the presumptive fin field even before fin buds were visible. Moreover, a perturbed expression pattern of bone morphogenetic protein 4 (bmp4), a marker of the pectoral fin fold, was observed in the developing fin buds of zebrafish embryos that lost the vdr function. These findings suggest that VDR signalling is crucial in the early stages of fin development, potentially influencing the process by regulating other signalling molecules such as Fgf24 and Bmp4.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peixe-Zebra
/
Receptores de Calcitriol
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Proteínas de Peixe-Zebra
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Proteína Morfogenética Óssea 4
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Nadadeiras de Animais
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Fatores de Crescimento de Fibroblastos
Limite:
Animals
Idioma:
En
Revista:
Anat Histol Embryol
Ano de publicação:
2024
Tipo de documento:
Article