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Human umbilical cord mesenchymal stem cells promote steroid-induced osteonecrosis of the femoral head repair by improving microvascular endothelial cell function.
Chen, Junwen; Jin, Wenyi; Zhong, Changheng; Cai, Wenxiang; Huang, Liangkun; Zhou, Jianlin; Peng, Hao.
Afiliação
  • Chen J; Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430062, China.
  • Jin W; Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430062, China.
  • Zhong C; Department of Biomedical Sciences, City University of Hong Kong, Kowloon Tong, Hong Kong 999077, Hong Kong Special Administrative Region.
  • Cai W; Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430062, China.
  • Huang L; Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430062, China.
  • Zhou J; Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430062, China.
  • Peng H; Department of Orthopedics, Renmin Hospital of Wuhan University, Wuhan 430062, China.
Aging (Albany NY) ; 16(9): 7928-7945, 2024 04 29.
Article em En | MEDLINE | ID: mdl-38696318
ABSTRACT
Recently, there has been growing interest in using cell therapy through core decompression (CD) to treat osteonecrosis of the femoral head (ONFH). Our study aimed to investigate the effectiveness and mechanism of human umbilical cord mesenchymal stem cells (hUCMSCs) in treating steroid-induced ONFH. We constructed a steroid-induced ONFH rabbit model as well as dexamethasone (Dex)-treated bone microvascular endothelial cells (BMECs) model of human femoral head. We injected hUCMSCs into the rabbit femoral head via CD. The effects of hUCMSCs on steroid-induced ONFH rabbit model and Dex-treated BMECs were evaluated via micro-CT, microangiography, histology, immunohistochemistry, wound healing, tube formation, and western blotting assay. Furthermore, we conducted single-cell RNA sequencing (scRNA-seq) to examine the characteristics of endothelial cells, the activation of signaling pathways, and inter-cellular communication in ONFH. Our data reveal that hUCMSCs improved the femoral head microstructure and bone repair and promoted angiogenesis in the steroid-induced ONFH rabbit model. Importantly, hUCMSCs improved the migration ability and angioplasty of Dex-treated BMECs by secreting COL6A2 to activate FAK/PI3K/AKT signaling pathway via integrin α1ß1.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dexametasona / Células Endoteliais / Transplante de Células-Tronco Mesenquimais / Necrose da Cabeça do Fêmur / Células-Tronco Mesenquimais Limite: Animals / Humans Idioma: En Revista: Aging (Albany NY) Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dexametasona / Células Endoteliais / Transplante de Células-Tronco Mesenquimais / Necrose da Cabeça do Fêmur / Células-Tronco Mesenquimais Limite: Animals / Humans Idioma: En Revista: Aging (Albany NY) Ano de publicação: 2024 Tipo de documento: Article