Properties of penicillin amidase immobilized by copolymerization with acrylamide.
Biotechnol Bioeng
; 21(9): 1543-52, 1979 Sep.
Article
em En
| MEDLINE
| ID: mdl-387106
ABSTRACT
An enzyme preparation in a spherical granule form was obtained by copolymerization of penicillin amidase (EC 3.5.1.11) (previously modified with maleic anhydride) and acrylamide via a crosslinking agent. As compared with the native enzyme, immobilized amidase is more resistant to heating, has a lower affinity to benzylpenicillin, and is less inhibited by phenylacetate. Its substrate specificity and optimum pH remain unchanged.
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Coleções:
01-internacional
Contexto em Saúde:
3_ND
Base de dados:
MEDLINE
Assunto principal:
Penicilina Amidase
/
Acrilamidas
/
Enzimas Imobilizadas
/
Amidoidrolases
Idioma:
En
Revista:
Biotechnol Bioeng
Ano de publicação:
1979
Tipo de documento:
Article