Evaluation of Sonicate Fluid Culture Cutoff Points for Periprosthetic Joint Infection Diagnosis.
Open Forum Infect Dis
; 11(5): ofae159, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38715572
ABSTRACT
Introduction:
Implant sonication is useful for recovery of periprosthetic joint infection (PJI) pathogens in culture, but exact cutoff points for definition of clinically significant sonicate fluid culture results vary from study to study. The aim of this study was to define ideal sonicate fluid culture cutoff points for PJI diagnosis.Methods:
Sonicate fluid cultures from hip and knee prosthesis components removed between February 2007 and December 2020 were studied. Prosthesis components were placed in solid containers in the operating room; in the clinical microbiology laboratory, 400â mL Ringer's solution was added, and containers subjected to vortexing, sonication and then vortexing, followed by centrifugation. Concentrated sonicate fluid was plated on aerobic and anaerobic solid media, and culture results reported semiquantitatively, as no growth, <20, 20-50, 51-100, or >100â CFU/10â mL sonicate fluid. Sonicate cultures from cement spacers and cultures yielding more than 1 microorganism were excluded. Sensitivity and specificity of each cutoff point was evaluated.Results:
A total of 1448 sonicate fluid cultures were evaluated, 68% from knees and 32% from hips. PJI was present in 644 (44%) cases. Sensitivity of sonicate culture was 75.0% at <20â CFU/10â mL, 55.3% at ≥20â CFU/10â mL, 46.9% at >51â CFU/10â mL, and 39.8% at >100â CFU/10â mL. Specificity was 78.2%, 99.8%, 100%, and 100%, at the 4 cutoff points, respectively.Conclusions:
A cutoff point for sonicate fluid culture positivity of ≥20â CFU/10â mL is suitable for PJI diagnosis.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Open Forum Infect Dis
Ano de publicação:
2024
Tipo de documento:
Article