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Characterization of putative calcium-dependent protein kinase-1 (TaCPK-1) gene: hubs in signalling and tolerance network of wheat under terminal heat.
Kumar, Ranjeet R; Niraj, Ravi K; Goswami, Suneha; Thimmegowda, Vinutha; Mishra, Gyan P; Mishra, Dwijesh; Rai, Gyanendra K; Kumar, Soora Naresh; Viswanathan, Chinnusamy; Tyagi, Aruna; Singh, Gyanendra P; Rai, Anil K.
Afiliação
  • Kumar RR; Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110012 India.
  • Niraj RK; Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110012 India.
  • Goswami S; Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110012 India.
  • Thimmegowda V; Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110012 India.
  • Mishra GP; Division of Seed Technology, ICAR-Indian Agricultural Research Institute, New Delhi, 110012 India.
  • Mishra D; Centre for Agricultural Bioinformatics (CABin), ICAR-Indian Agricultural Statistics Research Institute, New Delhi, 110012 India.
  • Rai GK; Sher-E-Kashmir University of Agricultural Science and Technology, Chatta, Jammu, 180009 India.
  • Kumar SN; CESCRA, Indian Agricultural Research Institute, New Delhi, 110012 India.
  • Viswanathan C; Division of Plant Physiology, Indian Agricultural Research Institute, New Delhi, 110012 India.
  • Tyagi A; Division of Biochemistry, Indian Agricultural Research Institute, New Delhi, 110012 India.
  • Singh GP; ICAR-National Bureau of Plant Genetic Resources, New Delhi, 110012 India.
  • Rai AK; Centre for Agricultural Bioinformatics (CABin), ICAR-Indian Agricultural Statistics Research Institute, New Delhi, 110012 India.
3 Biotech ; 14(6): 150, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38725866
ABSTRACT
Calcium-dependent protein kinase (CDPK) is member of one of the most important signalling cascades operating inside the plant system due to its peculiar role as thermo-sensor. Here, we identified 28 full length putative CDPKs from wheat designated as TaCDPK (1-28). Based on digital gene expression, we cloned full length TaCPK-1 gene of 1691 nucleotides with open reading frame (ORF) of 548 amino acids (accession number OP125853). The expression of TaCPK-1 was observed maximum (3.1-fold) in leaf of wheat cv. HD2985 (thermotolerant) under T2 (38 ± 3 °C, 2 h), as compared to control. A positive correlation was observed between the expression of TaCPK-1 and other stress-associated genes (MAPK6, CDPK4, HSFA6e, HSF3, HSP17, HSP70, SOD and CAT) involved in thermotolerance. Global protein kinase assay showed maximum activity in leaves, as compared to root, stem and spike under heat stress. Immunoblot analysis showed abundance of CDPK protein in wheat cv. HD2985 (thermotolerant) in response to T2 (38 ± 3 °C, 2 h), as compared to HD2329 (thermosusceptible). Calcium ion (Ca2+), being inducer of CDPK, showed strong Ca-signature in the leaf tissue (Ca-622 ppm) of thermotolerant wheat cv. under heat stress, whereas it was minimum (Ca-201 ppm) in spike tissue. We observed significant variations in the ionome of wheat under HS. To conclude, TaCPK-1 plays important role in triggering signaling network and in modulation of HS-tolerance in wheat. Supplementary Information The online version contains supplementary material available at 10.1007/s13205-024-03989-6.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: 3 Biotech Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: 3 Biotech Ano de publicação: 2024 Tipo de documento: Article