Coupling of surface plasmon resonance and mass spectrometry for molecular interaction studies in drug discovery.
Drug Discov Today
; 29(7): 104027, 2024 Jul.
Article
em En
| MEDLINE
| ID: mdl-38762085
ABSTRACT
Various analytical technologies have been developed for the study of target-ligand interactions. The combination of these technologies gives pivotal information on the binding mechanism, kinetics, affinity, residence time, and changes in molecular structures. Mass spectrometry (MS) offers structural information, enabling the identification and quantification of target-ligand interactions. Surface plasmon resonance (SPR) provides kinetic information on target-ligand interaction in real time. The coupling of MS and SPR complements each other in the studies of target-ligand interactions. Over the last two decades, the capabilities and added values of SPR-MS have been reported. This review summarizes and highlights the benefits, applications, and potential for further research of the SPR-MS approach.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Espectrometria de Massas
/
Ressonância de Plasmônio de Superfície
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Descoberta de Drogas
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Drug Discov Today
Ano de publicação:
2024
Tipo de documento:
Article