Effects of diet and antibiotics on anastomotic healing: A mouse model study with varied dietary fiber and fat, and pre-operative antibiotics.
Am J Surg
; 235: 115766, 2024 Sep.
Article
em En
| MEDLINE
| ID: mdl-38777716
ABSTRACT
INTRODUCTION:
This study investigated the separate impacts of diet and pre-operative antibiotics on gut microbiome and colonic anastomotic healing using a mouse model.METHODS:
Male C57BL/6J mice were fed either low-fat-high-fibre (SD) or high-fat-low-fiber (WD) groups for 6 weeks, then further received either pre-operative antibiotics or a control sham before a colonic anastomotic procedure was performed. After 7 days, the anastomosis was assessed and microbiota composition and biodiversity were analyzed in anastomotic tissue and stool.RESULTS:
WD-fed mice had shorter survival (5.2 â± â2.3 vs. 6.9 â± â2.3 days, p â= â0.022), increased weight loss (5.55 â± â3.80g vs. 2.65 â± â2.36g, p â= â0.03), and reduced biodiversity compared to SD-fed mice. Pre-operative antibiotics improved anastomotic healing scores (1.33 â± â0.65 vs. 2.08 â± â0.79, p â= â0.02) and reduced Enterococcus faecalis growth in tissue and stool (p â= â0.02, p â= â0.02). Improved anastomotic healing correlated with lower Enterococcus abundance (p â= â0.04) and higher collagen III and IV levels (p â= â0.01, 0.04) in anastomotic tissue.CONCLUSION:
SD promotes enhanced post-operative recovery and increased microbiome biodiversity, while pre-operative antibiotics enhance anastomotic healing by suppressing Enterococcus faecalis growth, mitigating collagen III/IV degradation.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cicatrização
/
Anastomose Cirúrgica
/
Fibras na Dieta
/
Colo
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Microbioma Gastrointestinal
/
Camundongos Endogâmicos C57BL
/
Antibacterianos
Limite:
Animals
Idioma:
En
Revista:
Am J Surg
Ano de publicação:
2024
Tipo de documento:
Article