Detecting 2'-5'-adenosine linked nucleic acids via acylation of secondary hydroxy functionality.
Bioorg Med Chem Lett
; 109: 129847, 2024 Sep 01.
Article
em En
| MEDLINE
| ID: mdl-38857849
ABSTRACT
2'-5'-Adenosine linked nucleic acids are crucial components in living cells that play significant roles, including participating in antiviral defense mechanisms by facilitating the breakdown of viral genetic material. In this report, we present a chemical derivatization method employing 5-fluoro-2-pyridinoyl-imidazole as the acylation agent, a strategy that can be effectively combined with advanced analytical tools, including Nuclear Magnetic Resonance spectroscopy and Liquid Chromatography-Mass Spectrometry, to enhance the characterization and detection capabilities. This marks the first instance of a simple method designed to detect 2'-5'-adenosine linked nucleic acids. The new method is characterized by its time-saving nature, simplicity, and relative accuracy compared to previous methods.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Adenosina
Idioma:
En
Revista:
Bioorg Med Chem Lett
Ano de publicação:
2024
Tipo de documento:
Article