MNase-Seq Analysis for Identifying Stress-Altered Nucleosome Occupancy in Plants.
Methods Mol Biol
; 2832: 33-46, 2024.
Article
em En
| MEDLINE
| ID: mdl-38869785
ABSTRACT
Nucleosome occupancy plays an important role in chromatin compaction, affecting biological processes by hampering the binding of cis-acting elements such as transcription factors, RNA polymerase machinery, and coregulatory. Accessible regions allow for cis-acting elements to bind DNA and regulate transcription. Here, we detail our protocol to profile nucleosome occupancy and chromatin structure dynamics under drought stress at the genome-wide scale using micrococcal nuclease (MNase) digestion. Combining variable MNase concentration treatments and high-throughput sequencing, we investigate the changes in the overall chromatin state using bread wheat samples from an exemplary drought experiment.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Estresse Fisiológico
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Triticum
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Nucleossomos
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Sequenciamento de Nucleotídeos em Larga Escala
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Nuclease do Micrococo
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2024
Tipo de documento:
Article