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PLASMA PROTEIN ELECTROPHORESIS IN THE WHITE STORK (CICONIA CICONIA): AGREEMENT BETWEEN AGAROSE GEL VERSUS CAPILLARY ZONE METHODS AND DEVELOPMENT OF REFERENCE INTERVALS.
Thorel, Milan; Roman, Yannick; Leclerc, Antoine.
Afiliação
  • Thorel M; ZooParc de Beauval & Beauval Nature, 41110 Saint-Aignan, France.
  • Roman Y; Le Parc de Clères, Département de Seine Maritime, 76690 Clères, France.
  • Leclerc A; ZooParc de Beauval & Beauval Nature, 41110 Saint-Aignan, France, antoine.leclerc@zoobeauval.com.
J Zoo Wildl Med ; 55(2): 330-340, 2024 Jun.
Article em En | MEDLINE | ID: mdl-38875190
ABSTRACT
The white stork (Ciconia ciconia) is a ciconiiform species widely represented in zoological institutions. Plasma protein electrophoresis is widely used in avian patients for assessment of inflammatory conditions, but reference intervals for this testing modality are lacking for the white stork. The two main electrophoretic methods are agarose gel electrophoresis (AGE) and capillary zone electrophoresis (CZE). This study assessed fresh plasma samples of healthy adult white storks (n = 30). Statistical analyses were performed to evaluate agreement between AGE and CZE. Typical electrophoretic fractions were obtained from both methods (prealbumin, albumin, α1, α2, ß, γ1, and γ2). The AGE and CZE methods were not equivalent for determining major electrophoretic fractions (except ß-globulins) and albuminglobulin ratio on plasma samples. An additional prealbumin fraction was seen with CZE. Reference intervals were established for each method as the smallest n group was 27 individuals for a given value; most values had normal distribution, and robust or parametric methods were used on the data.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aves / Proteínas Sanguíneas / Eletroforese Capilar / Eletroforese em Gel de Ágar Limite: Animals Idioma: En Revista: J Zoo Wildl Med Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Aves / Proteínas Sanguíneas / Eletroforese Capilar / Eletroforese em Gel de Ágar Limite: Animals Idioma: En Revista: J Zoo Wildl Med Ano de publicação: 2024 Tipo de documento: Article