Engineering Electron Transfer Pathway of Cytochrome P450s.
Molecules
; 29(11)2024 May 24.
Article
em En
| MEDLINE
| ID: mdl-38893355
ABSTRACT
Cytochrome P450s (P450s), a superfamily of heme-containing enzymes, existed in animals, plants, and microorganisms. P450s can catalyze various regional and stereoselective oxidation reactions, which are widely used in natural product biosynthesis, drug metabolism, and biotechnology. In a typical catalytic cycle, P450s use redox proteins or domains to mediate electron transfer from NAD(P)H to heme iron. Therefore, the main factors determining the catalytic efficiency of P450s include not only the P450s themselves but also their redox-partners and electron transfer pathways. In this review, the electron transfer pathway engineering strategies of the P450s catalytic system are reviewed from four aspects cofactor regeneration, selection of redox-partners, P450s and redox-partner engineering, and electrochemically or photochemically driven electron transfer.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oxirredução
/
Engenharia de Proteínas
/
Sistema Enzimático do Citocromo P-450
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Molecules
Ano de publicação:
2024
Tipo de documento:
Article