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Regulation of Osteosarcoma Cell Proliferation, Migration, and Invasion by miR-143 and miR-199a Through COX-2 Targeting.
Bixin, Huang; Yuling, Zheng; Ying, Mai; Jinming, Chen; Zhang, Zhongqi.
Afiliação
  • Bixin H; Department of Anesthesiology, The Affiliated Shunde Hospital of Jinan University, Foshan, China.
  • Yuling Z; Department of Anesthesiology, The Affiliated Shunde Hospital of Jinan University, Foshan, China.
  • Ying M; Department of Anesthesiology, The Affiliated Shunde Hospital of Jinan University, Foshan, China.
  • Jinming C; Department of Anesthesiology, The Affiliated Shunde Hospital of Jinan University, Foshan, China.
  • Zhang Z; Department of Anesthesiology, The Affiliated Shunde Hospital of Jinan University, Foshan, China.
Dose Response ; 22(2): 15593258241264947, 2024.
Article em En | MEDLINE | ID: mdl-38912334
ABSTRACT

Objective:

To investigate the biological role of miR-143 and miR-199a in mediating the progression of osteosarcoma (OS) by targeting cyclooxygenase (COX-2).

Introduction:

COX-2 plays a crucial role in the development and progression of OS. However, the specific regulatory mechanisms of COX-2 in OS are still not well understood.

Methods:

The expression levels of COX-2, miR-143 and miR-199a in OS tissues were detected using immunohistochemistry, qPCR, or western blot assays. The targeting relationship between miRNAs and COX-2 was determined. The effect of miRNA and COX-2 on OS cells was evaluated in vitro and in vivo.

Results:

COX-2 expression was upregulated while miR-143 and miR-199a were downregulated in OS tissues. miR-143 and miR-199a suppressed the proliferation, migration, and invasion of OS cells. The dual-luciferase reporter gene assay showed that COX-2 was a direct target of miR-143 and miR-199a. Genetic knockdown of COX-2 significantly suppressed cell proliferation, induced apoptosis, and inhibited migration and invasion of OS cells. The expression levels of COX-2 and PGE2 were decreased after the overexpression of miR-143 and miR-199a. Additionally, COX-2 silencing inhibited the tumorigenesis of OS and the synthesis of PGE2 in vivo.

Conclusions:

miR-143 and miR-199a/COX-2 axis modulates the proliferation, invasion, and migration in osteosarcoma.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Dose Response Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Dose Response Ano de publicação: 2024 Tipo de documento: Article