Bovine oocyte activation with bull or human sperm by conventional ICSI and Piezo-ICSI: Its relationship with PLCÉ activity.
Open Vet J
; 14(5): 1191-1198, 2024 May.
Article
em En
| MEDLINE
| ID: mdl-38938440
ABSTRACT
Background:
The intracytoplasmic sperm injection (ICSI) technique has low efficiency in cattle. This has mainly been attributed to the oocyte activation failure due to oocyte and/or sperm factors.Aim:
Our aim was to evaluate the effect of conventional ICSI and Piezo-ICSI with bull or human sperm on bovine oocyte activation and embryo development and to assess its relationship with the phospholipase C zeta (PLCÉ) activity of both species.Methods:
In vitro matured bovine oocytes were randomly divided into five groups and were fertilized as follows conventional ICSI using bovine sperm with chemical activation (control), conventional ICSI using bovine sperm, Piezo-ICSI using bovine sperm, conventional ICSI using human sperm, and Piezo-ICSI using human sperm. PLCÉ activity was determined in bull and human sperm samples.Results:
Within the groups using bull sperm, the oocytes fertilized by conventional ICSI had the lowest values of 2 pronuclei (PN) formation and cleavage, Piezo-ICSI increased both percentages and ICSI + chemical activation presented the highest 2 PN, cleavage, and blastocyst rates (p < 0.05). Within the groups using human sperm, the oocytes fertilized by Piezo-ICSI presented higher 2 PN and cleavage rates than those activated by conventional ICSI (p < 0.05). Piezo-ICSI with human sperm increased bovine oocyte activation as much as conventional ICSI + chemical activation with bovine sperm (p < 0.05). Higher values of PLCÉ activity were found in human sperm compared with bovine sperm (p < 0.05).Conclusion:
Our results suggest that the higher stability of the bovine sperm in combination with its relatively low content of PLCÉ impairs bovine oocyte activation after ICSI.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Oócitos
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Espermatozoides
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Injeções de Esperma Intracitoplásmicas
Limite:
Animals
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Female
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Humans
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Male
Idioma:
En
Revista:
Open Vet J
Ano de publicação:
2024
Tipo de documento:
Article