Analyses of the Mx family members in lumpfish: Molecular characterization, phylogeny, and gene expression analyses.
Dev Comp Immunol
; 159: 105225, 2024 Oct.
Article
em En
| MEDLINE
| ID: mdl-38992732
ABSTRACT
Members of the myxovirus resistance (Mx) protein family play an essential role in antiviral immunity. They are Dynamin-like GTPases, induced by interferons. In the current study, we have characterized two predicted MX genes (MX1 and MX2) from lumpfish (Cyclopterus lumpus L.), having 12 and 13 exons, respectively. Mx2 has two isoforms (Mx2-X1 and Mx2-X2) which differ in exon 1. The lumpfish Mx proteins contain an N-terminal Dynamin-like GTPase domain, the middle domain (MD) and GTPase effector domain (GED) characteristic for Mx proteins. Phylogenetic analyses grouped all the lumpfish Mx sequences in group 1, and synteny analyses showed that both genes were localized at chromosome 5 in proximity to the genes Tohc7, Atxn7 and Psmd6. In vitro stimulation experiment showed that both MX1 and MX2-X2 were highly upregulated upon exposure to poly(IC), but not bacteria, 24 h post exposure, indicating their role in antiviral immunity.
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Texto completo:
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Filogenia
/
Proteínas de Peixes
/
Proteínas de Resistência a Myxovirus
Limite:
Animals
Idioma:
En
Revista:
Dev Comp Immunol
Ano de publicação:
2024
Tipo de documento:
Article