Triptonide protects retinal cells from oxidative damage via activation of Nrf2 signaling.
Int J Mol Med
; 54(3)2024 09.
Article
em En
| MEDLINE
| ID: mdl-38994762
ABSTRACT
Agerelated macular degeneration (AMD) is an ocular disease that threatens the visual function of older adults worldwide. Key pathological processes involved in AMD include oxidative stress, inflammation and choroidal vascular dysfunction. Retinal pigment epithelial cells and Müller cells are most susceptible to oxidative stress. Traditional herbal medicines are increasingly being investigated in the field of personalized medicine in ophthalmology. Triptonide (Tn) is a diterpene tricyclic oxide, the main active ingredient in the extract from the Chinese herbal medicinal plant Tripterygium wilfordii, and is considered an effective immunosuppressant and antiinflammatory drug. The present study investigated the potential beneficial role of Tn in retinal oxidative damage in order to achieve personalized treatment for early AMD. An oxidative stress model of retinal cells induced by H2O2 and a retinal injury model of mice induced by light and NMethylDaspartic acid were constructed. In vitro, JC1 staining, flow cytometry and apoptosis assay confirmed that low concentrations of Tn effectively protected retinal cells from oxidative damage, and reverse transcriptionquantitative PCR and western blotting analyses revealed that Tn reduced the expression of retinal oxidative stressrelated genes and inflammatory factors, which may depend on the PI3K/AKT/mTORinduced Nrf2 signaling pathway. In vivo, by retinal immunohistochemistry, hematoxylin and eosin staining and electroretinogram assay, it was found that retinal function and structure improved and choroidal neovascularization was significantly inhibited after Tn pretreatment. These results suggested that Tn is an efficient Nrf2 activator, which can be expected to become a new intervention for diseases such as AMD, to inhibit retinal oxidative stress damage and pathological neovascularization.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Retina
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Transdução de Sinais
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Estresse Oxidativo
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Fator 2 Relacionado a NF-E2
Limite:
Animals
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Humans
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Male
Idioma:
En
Revista:
Int J Mol Med
Ano de publicação:
2024
Tipo de documento:
Article