Automation and standardisation of a quantitative multiplex PCR assay using PCR.Ai.
J Virol Methods
; 329: 114981, 2024 Jul 14.
Article
em En
| MEDLINE
| ID: mdl-39004191
ABSTRACT
BACKGROUND:
We previously undertook a prospective clinical study to evaluate PCR.Ai's (www.pcr.ai) accuracy and impact when automating the manual data-analysis and quality control steps associated with routine clinical pathogen testing using a non-quantitative multiplex quantitative real-time PCR (qPCR). In this study we demonstrated 100â¯% concurrence between our manual routine analysis method and PCR.Ai. This paper expands the evaluation of PCR.Ai's (www.pcr.ai) accuracy and impact using a multiplex quantitative real-time PCR (qPCR).OBJECTIVES:
We evaluated the impact of PCR.Ai when used as the final interpretation/verification step for routine in-house multiplex quantitative qPCR tests for CMV, EBV and adenovirus from blood samples for a total of 1350 interpretations. STUDYDESIGN:
We compared PCR.Ai to our existing manual interpretation, to determine accuracy and hands on time savings. RESULTS ANDCONCLUSIONS:
There was 100â¯% concurrence between validated CMV, EBV and adenovirus detection and quantitation by our manual routine analysis method and PCR.Ai. Furthermore, there were significant routine savings with PCR.Ai of 63â¯minutes/ run. Our conclusion is that for quantitative tests PCR.Ai is a highly accurate time-saving tool that reduces complexity of qPCR analysis and hence the need for specialists and hands-on time. It demonstrated capabilities to enable us to get results out more quickly with lower costs and less risk of errors.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Virol Methods
Ano de publicação:
2024
Tipo de documento:
Article