Magic-angle spinning NMR structure of Opa60 in lipid bilayers.
J Struct Biol X
; 9: 100098, 2024 Jun.
Article
em En
| MEDLINE
| ID: mdl-39010882
ABSTRACT
Here we report the structure of Opa60 in lipid bilayers using proton-detected magic-angle spinning nuclear magnetic resonance (MAS NMR). Preparations including near-native oligosaccharide lipids reveal a consistent picture of a stable transmembrane beta barrel with a minor increase in the structured region as compared with the previously reported detergent structure. The large variable loops known to interact with host proteins could not be detected, confirming their dynamic nature even in a lipid bilayer environment. The structure provides a starting point for investigation of the functional role of Opa60 in gonococcal infection, which is understood to involve interaction with host proteins. At the same time, it demonstrates the recent advances in proton-detected methodology for membrane protein structure determination at atomic resolution by MAS NMR.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
J Struct Biol X
Ano de publicação:
2024
Tipo de documento:
Article