Epigenomic Profiling of B Cell Subsets by CUT&Tag.
Methods Mol Biol
; 2826: 65-77, 2024.
Article
em En
| MEDLINE
| ID: mdl-39017886
ABSTRACT
Epigenetic programs play a key role in regulating the development and function of immune cells. However, conventional methods for profiling epigenetic mechanisms, such as the post-translational modifications to histones, present several technical challenges that prevent a complete understanding of gene regulation. Here, we provide a detailed protocol of the Cleavage Under Targets and Tagmentation (CUT&Tag) chromatin profiling technique for identifying histone modifications in human and mouse lymphocytes.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Histonas
/
Subpopulações de Linfócitos B
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Epigênese Genética
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Epigenômica
Limite:
Animals
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Humans
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2024
Tipo de documento:
Article