Glycoside Phosphorylase Catalyzed Cellulose and ß-1,3-Glucan Synthesis Using Chromophoric Glycosyl Acceptors.
Biomacromolecules
; 25(8): 5048-5057, 2024 Aug 12.
Article
em En
| MEDLINE
| ID: mdl-39025475
ABSTRACT
Glycoside phosphorylases are enzymes that are frequently used for polysaccharide synthesis. Some of these enzymes have broad substrate specificity, enabling the synthesis of reducing-end-functionalized glucan chains. Here, we explore the potential of glycoside phosphorylases in synthesizing chromophore-conjugated polysaccharides using commercially available chromophoric model compounds as glycosyl acceptors. Specifically, we report cellulose and ß-1,3-glucan synthesis using 2-nitrophenyl ß-d-glucopyranoside, 4-nitrophenyl ß-d-glucopyranoside, and 2-methoxy-4-(2-nitrovinyl)phenyl ß-d-glucopyranoside with Clostridium thermocellum cellodextrin phosphorylase and Thermosipho africanus ß-1,3-glucan phosphorylase as catalysts. We demonstrate activity for both enzymes with all assayed chromophoric acceptors and report the crystallization-driven precipitation and detailed structural characterization of the synthesized polysaccharides, i.e., their molar mass distributions and various structural parameters, such as morphology, fibril diameter, lamellar thickness, and crystal form. Our results provide insights for the studies of chromophore-conjugated low molecular weight polysaccharides, glycoside phosphorylases, and the hierarchical assembly of crystalline cellulose and ß-1,3-glucan.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Celulose
/
Beta-Glucanas
/
Glucosiltransferases
Idioma:
En
Revista:
Biomacromolecules
Ano de publicação:
2024
Tipo de documento:
Article