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In vitro validation of helium ion irradiations as a function of linear energy transfer in radioresistant human malignant cells.
Fira, Aleksandra M Ristic; Keta, Otilija D; Petkovic, Vladana D; Dordevic, Milos; Petringa, Giada; Fattori, Serena; Catalano, Roberto; Cirrone, Giuseppe Pablo; Cuttone, Giacomo; Sakata, Dousatsu; Tran, Ngoc Hoang; Chatzipapas, Konstantinos; Incerti, Sebastien; Petrovic, Ivan M.
Afiliação
  • Fira AMR; Vinca Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia.
  • Keta OD; Vinca Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia.
  • Petkovic VD; Vinca Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia.
  • Dordevic M; Vinca Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia.
  • Petringa G; Istituto Nazionale di Fisica Nucleare, LNS, Catania, Italy.
  • Fattori S; Istituto Nazionale di Fisica Nucleare, LNS, Catania, Italy.
  • Catalano R; Istituto Nazionale di Fisica Nucleare, LNS, Catania, Italy.
  • Cirrone GP; Istituto Nazionale di Fisica Nucleare, LNS, Catania, Italy.
  • Cuttone G; Istituto Nazionale di Fisica Nucleare, LNS, Catania, Italy.
  • Sakata D; Division of Health Sciences, Osaka University, Osaka, Japan.
  • Tran NH; University of Bordeaux, CNRS, LP2I, UMR 5797, F-33170 Gradignan, France.
  • Chatzipapas K; University of Brest, Brest, France.
  • Incerti S; University of Bordeaux, CNRS, LP2I, UMR 5797, F-33170 Gradignan, France.
  • Petrovic IM; Vinca Institute of Nuclear Sciences, University of Belgrade, Belgrade, Serbia.
Int J Radiat Biol ; : 1-12, 2024 Jul 26.
Article em En | MEDLINE | ID: mdl-39058324
ABSTRACT

PURPOSE:

Based on considerable interest to enlarge the experimental database of radioresistant cells after their irradiation with helium ions, HTB140, MCF-7 and HTB177 human malignant cells are exposed to helium ion beams having different linear energy transfer (LET). MATERIALS AND

METHODS:

The cells are irradiated along the widened 62 MeV/u helium ion Bragg peak, providing LET of 4.9, 9.8, 23.4 and 36.8 keV/µm. Numerical simulations with the Geant4 toolkit are used for the experimental design. Cell survival is evaluated and compared with reference γ-rays. DNA double strand breaks are assessed via γ-H2AX foci.

RESULTS:

With the increase of LET, surviving fractions at 2 Gy decrease, while RBE (2 Gy, γ) gradually increase. For HTB140 cells, above the dose of 4 Gy, a slight saturation of survival is observed while the increase of RBE (2 Gy, γ) remains unaffected. With the increase of LET the increase of γ-H2AX foci is revealed at 0.5 h after irradiation. There is no significant difference in the number of foci between the cell lines for the same LET. From 0.5 to 24 h, the number of foci drops reaching its residual level. For each time point, there are small differences in DNA DSB among the three cell lines.

CONCLUSION:

Analyses of data acquired for the three cell lines irradiated by helium ions, having different LET, reveal high elimination capacity and creation of a large number of DNA DSB with respect to γ-rays, and are between those reported for protons and carbon ions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Int J Radiat Biol Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Int J Radiat Biol Ano de publicação: 2024 Tipo de documento: Article