Design of U-shaped peptides with long-lasting antifouling efficacy: Toward a feasible electrochemical aptasensor for robust detection in human serum.
Anal Chim Acta
; 1318: 342953, 2024 Aug 22.
Article
em En
| MEDLINE
| ID: mdl-39067928
ABSTRACT
BACKGROUND:
Developing biosensors with antifouling properties is essential for accurately detecting low-concentration biomarkers in complex biological matrix, which is imperative for effective disease diagnosis and treatment. Herein, an antifouling electrochemical aptasensor qualifying for probing targets in human serum was explored based on newly-devised peptides that could form inverted U-shaped structures with long-term stability.RESULTS:
The inverted U-shaped peptides (U-Pep) with two terminals of thiol groups grafted onto the Au-modified electrode showcase superior antifouling properties in terms of high stability against enzymatic hydrolysis and long acting against biofouling in actual biofluids. The construction of the outlined antifouling electrochemical aptasensor just involved the fabrication of Au-deposited poly(3,4 ethylenedioxythiophene) (Au/PEDOT) modified electrode, followed by one-step co-incubation in the peptides and the aptamer probes with the Au/PEDOT electrode. Taking a typical biomarker of alpha-fetoprotein (AFP) for detection, this elegant antifouling aptasenor demonstrated a nice response for probing the target AFP with a low detection limit of 0.27 pg/mL and a wide linear scope of 1.0 pg/mL to 1.0 µg/mL, and furthermore qualified for assaying of AFP in human serum samples with satisfactory accuracy and feasibility.SIGNIFICANCE:
This engineering strategy of U-Pep with long-lasting antifouling efficacy opens a new horizon for high-performance antifouling biosensors suitable for detection in complex bifluids, and it could spark more inspiration for a follow-up exploration of other featured antifouling biomaterials.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Peptídeos
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Técnicas Biossensoriais
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Aptâmeros de Nucleotídeos
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Técnicas Eletroquímicas
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Incrustação Biológica
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Ouro
Limite:
Humans
Idioma:
En
Revista:
Anal Chim Acta
Ano de publicação:
2024
Tipo de documento:
Article