Harnessing Viral Promoters for Targeted RNAi: Engineering Phloem-Specific Resistance Against Tomato Yellow Leaf Curl Thailand Virus.
Methods Mol Biol
; 2844: 239-245, 2024.
Article
em En
| MEDLINE
| ID: mdl-39068344
ABSTRACT
Phloem-specific promoter efficiently triggers graft-transmissible RNA interference (gtRNAi). We leveraged a phloem-specific promoter derived from the Rice tungro bacilliform virus, optimizing the RNAi mechanism's efficiency and specificity. Here, we detail the construction of phloem-specific promoter-based gtRNAi system and its application through grafting experiments, demonstrating its effectiveness in inducing tomato yellow leaf curl Thailand virus (TYLCHTV) resistance in non-transgenic scions. This strategy presents a practical application for protecting crops against viruses without genetically modifying the entire plant.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças das Plantas
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Regiões Promotoras Genéticas
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Interferência de RNA
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Begomovirus
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Floema
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Resistência à Doença
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2024
Tipo de documento:
Article