Protocol for next-generation sequencing of the LSD virus genome using an amplicon-based approach.
STAR Protoc
; 5(3): 103020, 2024 Jul 30.
Article
em En
| MEDLINE
| ID: mdl-39083382
ABSTRACT
Lumpy skin disease (LSD) is a viral disease predominantly affecting cattle caused by a poxvirus belonging to the capripoxvirus genus. Here, we present a protocol for next-generation sequencing of the LSD virus genome using an amplicon-based approach. We describe steps for DNA extraction, viral DNA enrichment, amplicon pooling and purification, and library preparation and pooling. We then detail procedures for sequencing and computational analysis. This protocol can be adapted to any Illumina sequencing platform as an accelerated and scalable system. For complete details on the use and execution of this protocol, please refer to Bhatt et al.1,2.
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1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
STAR Protoc
Ano de publicação:
2024
Tipo de documento:
Article