Biostimulation accelerates landfill stabilization and resource utilization efficiency, providing feasible technical support for the overall lifecycle management of landfills.

ABSTRACT

Although sanitary landfill is one of the principal municipal solid waste (MSW) treatment and disposal methods, its limitations, such as insufficient use of resources, long stability time, and high risk of environmental pollution, must be urgently resolved. The effect of multifunctional microbial community (MMC) inoculation on MSW landfill process was investigated using simulated anaerobic bioreactor landfill (ABL), and composition and microbial community structure of waste, leachate water quality, and gas production were monitored. MMC inoculation significantly accelerated lignocellulose degradation, and the (Hemicellulose content + Cellulose content)/Lignin content ((C + H)/L) of MMC inoculation treatment was 0.89 ± 0.04 on day 44, which was significantly lower than that of the control group (1.14 ± 0.02). At the end of the landfill process, the reductive organic matter, ammonia nitrogen, and volatile fatty acids in the leachate of the MMC group decreased to 9400.00 ± 288.68, 332.78 ± 5.77, and 79.33 ± 6.44 mg L-1, respectively, significantly lower than those of the control group (24,167.00 ± 208.17, 551.14 ± 5.60, and 156.33 ± 8.22 mg L-1). Meanwhile, MMC inoculation increased the methane production to 118.12 ± 5.42 L kg-1 of dry matter, significantly higher than the output of the control group (60.60 ± 2.24 L kg-1). MMC inoculation optimized the microbial community structure in ABL and increased lignocellulose-degrading microorganisms (Brevundimonas, Cellvibrio, Leifsonia, and Devosia) and methanogen (Methanosaeta and Methanoculleus) abundance in the middle stage of landfill. Moreover, MMC introduction improved the abundance of carbon metabolism enzymes and increased saprophytic fungal abundance by 30.09% in the middle stage of landfill. Overall, these findings may help in developing an effective method to increase the lifespan of landfills and enhance their post-closure management.