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Caulerpa chemnitzia polysaccharide exerts immunomodulatory activity in macrophages by mediating the succinate/PHD2/HIF-1α/IL-1ß pathway.
Zeng, Jinzi; Liu, Jun; Zhao, Ning; Wong, Io Nam; Huang, Riming.
Afiliação
  • Zeng J; Guangdong Provincial Key Laboratory of Food Quality and Safety/College of Food Science, South China Agricultural University, Guangzhou 510642, China.
  • Liu J; Laboratory of Pathogenic Biology, Guangdong Medical University, Zhanjiang 524023, China.
  • Zhao N; Shenzhen Hospital of Integrated Traditional Chinese and Western Medicine, Shenzhen 518104, China.
  • Wong IN; Faculty of Medicine, Macau University of Science and Technology, Macau 999078, Macau. Electronic address: inwong@must.edu.mo.
  • Huang R; Guangdong Provincial Key Laboratory of Food Quality and Safety/College of Food Science, South China Agricultural University, Guangzhou 510642, China. Electronic address: huangriming@scau.edu.cn.
Int J Biol Macromol ; 277(Pt 3): 134450, 2024 Aug 03.
Article em En | MEDLINE | ID: mdl-39098690
ABSTRACT
Algal polysaccharide is an important food functional factor with diverse bioactive and low toxicity. Previous studies have confirmed Caulerpa chemnitzia polysaccharides (CRVP) have immunomodulatory activity, but the immunomodulatory mechanism of CRVP in macrophages has not been thoroughly explored yet. In our research, we found that CRVP has outstanding immunomodulatory activity in macrophages, which is reflected in promoting cell proliferation, upregulating cytokines (IL-1ß, IL-6, and TNF-α) expression, and increasing NO and ROS levels. Additionally, the result of joint analysis of untargeted metabolomics showed metabolism played a major role in the immunomodulatory of CRVP and suggested succinic acid was a key metabolite. Further verification indicated that the accumulation of succinic acid in macrophages after administered with CRVP, induced the down-regulation of prolyl hydroxylase domain 2 (PHD2) and up-regulation of hypoxia-inducible factor-1α (HIF-1α), thereby enhancing IL-1ß expression. Together, the immunomodulatory activity of CRVP in macrophages via succinate/PHD2/HIF-1α/IL-1ß pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Int J Biol Macromol Ano de publicação: 2024 Tipo de documento: Article