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Animal and organoid models to elucidate the anti-fibrotic effect of steroid on biliary atresia.
Liu, Fangran; Lui, Vincent Chi Hang; Wu, Zhongluan; Blakeley, Paul David; Tang, Clara Sze Man; Tam, Paul Kwong Hang; Wong, Kenneth Kak Yuen; Chung, Patrick Ho Yu.
Afiliação
  • Liu F; Department of Surgery, School of Clinical Medicine, Queen Mary Hospital, The University of Hong Kong, 102 Pokfulam Road, Hong Kong SAR, China.
  • Lui VCH; Department of Surgery, School of Clinical Medicine, Queen Mary Hospital, The University of Hong Kong, 102 Pokfulam Road, Hong Kong SAR, China.
  • Wu Z; Dr. Li Dak-Sum Research Centre, The University of Hong Kong, Hong Kong SAR, China.
  • Blakeley PD; Department of Surgery, School of Clinical Medicine, Queen Mary Hospital, The University of Hong Kong, 102 Pokfulam Road, Hong Kong SAR, China.
  • Tang CSM; Department of Surgery, School of Clinical Medicine, Queen Mary Hospital, The University of Hong Kong, 102 Pokfulam Road, Hong Kong SAR, China.
  • Tam PKH; Department of Surgery, School of Clinical Medicine, Queen Mary Hospital, The University of Hong Kong, 102 Pokfulam Road, Hong Kong SAR, China.
  • Wong KKY; Dr. Li Dak-Sum Research Centre, The University of Hong Kong, Hong Kong SAR, China.
  • Chung PHY; Faculty of Medicine, Macau University of Science and Technology, Macau SAR, China.
Pediatr Surg Int ; 40(1): 214, 2024 Aug 05.
Article em En | MEDLINE | ID: mdl-39102048
ABSTRACT

PURPOSE:

We performed animal and organoid study to evaluate the anti-fibrotic effect of steroid on biliary atresia (BA) and the underlying patho-mechanism.

METHODS:

BA animal models were created by inoculation of mice on post-natal day 1 with rhesus rotavirus (RRV). They received either 20 µl phosphate-buffered saline (PBS) or steroid from day 21 to day 34. On day 34, their serum samples were collected for hormonal markers. Necrosis, fibrosis and CK 19 expression in the liver were evaluated. Liver organoids were developed and their morphology as well as bulk RNA sequencing data were analyzed.

RESULTS:

Twenty-four mice developed BA features after RRV injection and were equally divided into steroid and PBS groups. On day 34, the weight gain of steroid group increased significantly than PBS group (p < 0.0001). All mice in the PBS group developed liver fibrosis but only one mouse in the steroid group did. Serum bilirubin and liver parenchymal enzymes were significantly lower in steroid group. The morphology of liver organoids were different between the two groups. A total of 6359 differentially expressed genes were found between steroid group and PBS group.

CONCLUSION:

Based on our findings obtained from RRV-induced BA animal and organoid models, steroid has the potential to mitigate liver fibrosis in BA.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Atresia Biliar / Organoides / Modelos Animais de Doenças / Cirrose Hepática Limite: Animals Idioma: En Revista: Pediatr Surg Int Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Atresia Biliar / Organoides / Modelos Animais de Doenças / Cirrose Hepática Limite: Animals Idioma: En Revista: Pediatr Surg Int Ano de publicação: 2024 Tipo de documento: Article