Action of a cysteine proteinase from pupae of the blowfly Aldrichina grahami on the oxidized B-chain of bovine insulin.
Comp Biochem Physiol B
; 82(4): 721-4, 1985.
Article
em En
| MEDLINE
| ID: mdl-3912099
ABSTRACT
A cysteine proteinase purified from pupae of the blowfly (A. grahami) was tested for its peptide-bond specificity against the oxidized B-chain of insulin. Fifteen peptides were separated on HPLC using both gradient and isocratic elution methods. Analyses of amino acid content and N-terminal amino acids indicated that these were eleven homogeneous peptides produced by digestion and undigested insulin B-chain. Glu13-Ala14 and Tyr26-Thr27 were the major cleavage sites, and Asn3-Gln4, Cys7-Gly8, Tyr16-Leu17, Leu17-Val18 and Cys19-Gly20 were also often cleaved. These findings show the similarity between this enzyme and cathepsin L.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Endopeptidases
/
Himenópteros
/
Insulina
Limite:
Animals
Idioma:
En
Revista:
Comp Biochem Physiol B
Ano de publicação:
1985
Tipo de documento:
Article