Structural insights into the cooperative nucleosome recognition and chromatin opening by FOXA1 and GATA4.
Mol Cell
; 84(16): 3061-3079.e10, 2024 Aug 22.
Article
em En
| MEDLINE
| ID: mdl-39121853
ABSTRACT
Mouse FOXA1 and GATA4 are prototypes of pioneer factors, initiating liver cell development by binding to the N1 nucleosome in the enhancer of the ALB1 gene. Using cryoelectron microscopy (cryo-EM), we determined the structures of the free N1 nucleosome and its complexes with FOXA1 and GATA4, both individually and in combination. We found that the DNA-binding domains of FOXA1 and GATA4 mainly recognize the linker DNA and an internal site in the nucleosome, respectively, whereas their intrinsically disordered regions interact with the acidic patch on histone H2A-H2B. FOXA1 efficiently enhances GATA4 binding by repositioning the N1 nucleosome. In vivo DNA editing and bioinformatics analyses suggest that the co-binding mode of FOXA1 and GATA4 plays important roles in regulating genes involved in liver cell functions. Our results reveal the mechanism whereby FOXA1 and GATA4 cooperatively bind to the nucleosome through nucleosome repositioning, opening chromatin by bending linker DNA and obstructing nucleosome packing.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ligação Proteica
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Nucleossomos
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Microscopia Crioeletrônica
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Fator de Transcrição GATA4
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Fator 3-alfa Nuclear de Hepatócito
Limite:
Animals
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Humans
Idioma:
En
Revista:
Mol Cell
Ano de publicação:
2024
Tipo de documento:
Article