Rapamycin-Induced Translocation of Meiotic Nuclear Proteins in Saccharomyces cerevisiae.
Methods Mol Biol
; 2818: 213-226, 2024.
Article
em En
| MEDLINE
| ID: mdl-39126477
ABSTRACT
Conditional depletion of proteins is a potential strategy to elucidate protein function, especially in complex cellular processes like meiosis. Several methods are available to effectively deplete a protein in a conditional manner. Conditional loss of a protein function can be achieved by depleting it from its region of action by degrading it. A conditional loss of protein function can also be achieved by sequestering it to a functionally unavailable compartment inside the cell. This chapter describes anchor away, a conditional depletion tool that can deplete a protein both temporally and spatially by translocation. It utilizes the affinity of FRB to bind FKBP12 in the presence of rapamycin for a quick and efficient translocation of the protein to a designated location. Anchor away is a reliable tool for the study of meiotic proteins, as only small quantities of rapamycin are required to efficiently and rapidly translocate the protein of interest without compromising meiotic progression.
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MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Sirolimo
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Transporte Proteico
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Proteínas de Saccharomyces cerevisiae
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Meiose
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2024
Tipo de documento:
Article