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The molecular architecture of the nuclear basket.
Singh, Digvijay; Soni, Neelesh; Hutchings, Joshua; Echeverria, Ignacia; Shaikh, Farhaz; Duquette, Madeleine; Suslov, Sergey; Li, Zhixun; van Eeuwen, Trevor; Molloy, Kelly; Shi, Yi; Wang, Junjie; Guo, Qiang; Chait, Brian T; Fernandez-Martinez, Javier; Rout, Michael P; Sali, Andrej; Villa, Elizabeth.
Afiliação
  • Singh D; School of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Soni N; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Hutchings J; School of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Echeverria I; Department of Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA 94158, USA.
  • Shaikh F; School of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Duquette M; School of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Suslov S; School of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA.
  • Li Z; State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, School of Life Sciences, Peking University, Beijing 100871, P.R. China.
  • van Eeuwen T; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY 10065, USA.
  • Molloy K; Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY 10065, USA.
  • Shi Y; Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY 10065, USA.
  • Wang J; Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY 10065, USA.
  • Guo Q; State Key Laboratory of Protein and Plant Gene Research, Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, School of Life Sciences, Peking University, Beijing 100871, P.R. China.
  • Chait BT; Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, The Rockefeller University, New York, NY 10065, USA.
  • Fernandez-Martinez J; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY 10065, USA; Ikerbasque, Basque Foundation for Science, 48013 Bilbao, Spain; Instituto Biofisika (UPV/EHU, CSIC), University of the Basque Country, 48940 Leioa, Spain. Electronic address: javier.fernandezma@ehu.eu
  • Rout MP; Laboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY 10065, USA. Electronic address: rout@rockefeller.edu.
  • Sali A; Department of Bioengineering and Therapeutic Sciences, University of California, San Francisco, San Francisco, CA 94158, USA; Quantitative Biosciences Institute, University of California, San Francisco, San Francisco, CA 94158, USA; Department of Pharmaceutical Chemistry, University of California, S
  • Villa E; School of Biological Sciences, University of California, San Diego, La Jolla, CA 92093, USA; Howard Hughes Medical Institute, University of California, San Diego, La Jolla, CA 92093, USA. Electronic address: evilla@ucsd.edu.
Cell ; 187(19): 5267-5281.e13, 2024 Sep 19.
Article em En | MEDLINE | ID: mdl-39127037
ABSTRACT
The nuclear pore complex (NPC) is the sole mediator of nucleocytoplasmic transport. Despite great advances in understanding its conserved core architecture, the peripheral regions can exhibit considerable variation within and between species. One such structure is the cage-like nuclear basket. Despite its crucial roles in mRNA surveillance and chromatin organization, an architectural understanding has remained elusive. Using in-cell cryo-electron tomography and subtomogram analysis, we explored the NPC's structural variations and the nuclear basket across fungi (yeast; S. cerevisiae), mammals (mouse; M. musculus), and protozoa (T. gondii). Using integrative structural modeling, we computed a model of the basket in yeast and mammals that revealed how a hub of nucleoporins (Nups) in the nuclear ring binds to basket-forming Mlp/Tpr proteins the coiled-coil domains of Mlp/Tpr form the struts of the basket, while their unstructured termini constitute the basket distal densities, which potentially serve as a docking site for mRNA preprocessing before nucleocytoplasmic transport.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Poro Nuclear / Transporte Ativo do Núcleo Celular / Complexo de Proteínas Formadoras de Poros Nucleares Limite: Animals Idioma: En Revista: Cell Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Saccharomyces cerevisiae / Poro Nuclear / Transporte Ativo do Núcleo Celular / Complexo de Proteínas Formadoras de Poros Nucleares Limite: Animals Idioma: En Revista: Cell Ano de publicação: 2024 Tipo de documento: Article