Mutagenic effects of ultraviolet radiation and trimethyl psoralen in mycoplasma toward a minimal genome.
Genes Genet Syst
; 992024 Oct 18.
Article
em En
| MEDLINE
| ID: mdl-39135235
ABSTRACT
Mycoplasmas, autonomously culturable bacteria with the smallest genome, are an important organism to understand the minimal form of life. Mutagenesis using mutagens is a useful methodology for understanding the essential regions of genomic information. Ultraviolet light (UV) and trimethyl psoralen (TMP) are mutagens known to induce various mutations; the latter is reported to specifically induce deletions in nematodes. However, their mutagenic effects on mycoplasma are not known. Here, we exposed Metamycoplasma salivarium to UV-C light or TMP and UV-A as mutagens, and analyzed the mutational pattern after serial cultivation ranging from 34 to 56 rounds for different lineages. Our results showed that more deletions, but fewer point mutations, were induced with TMP and UV-A than with UV-C, indicating the usefulness of TMP in inducing deletions. In addition, we compared our results with mutational data from other studies, which suggested that the combination of TMP and UV-A or UV-C exposure both induced point mutations that were highly biased toward CâT and GâA transitions. These data provide useful basic knowledge for mutational studies on M. salivarium.
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01-internacional
Base de dados:
MEDLINE
Assunto principal:
Raios Ultravioleta
/
Genoma Bacteriano
Idioma:
En
Revista:
Genes Genet Syst
Ano de publicação:
2024
Tipo de documento:
Article