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Heterotypic interaction promotes asymmetric division of human hematopoietic progenitors.
Candelas, Adrian; Vianay, Benoit; Gelin, Matthieu; Faivre, Lionel; Larghero, Jerome; Blanchoin, Laurent; Théry, Manuel; Brunet, Stéphane.
Afiliação
  • Candelas A; Human Immunology, Pathophysiology, Immunotherapy, INSERM Unit 976, Institut de Recherche St Louis, AP-HP, Hôpital Saint-Louis, Université Paris Cité, F-75010 Paris, France.
  • Vianay B; Cytomorpholab, University Grenoble-Alpes, CEA, CNRS, INRA, Laboratoire de Phyiologie Cellulaire & Végétale, F-38054 Grenoble, France.
  • Gelin M; Human Immunology, Pathophysiology, Immunotherapy, INSERM Unit 976, Institut de Recherche St Louis, AP-HP, Hôpital Saint-Louis, Université Paris Cité, F-75010 Paris, France.
  • Faivre L; Unité de Thérapie Cellulaire, Human Immunology, Pathophysiology, Immunotherapy, INSERM Unit 976, AP-HP, Hôpital Saint-Louis, Center of Clinical Investigations in Biotherapies of Cancer CBT501, Université Paris Cité, F-75010 Paris, France.
  • Larghero J; Unité de Thérapie Cellulaire, Human Immunology, Pathophysiology, Immunotherapy, INSERM Unit 976, AP-HP, Hôpital Saint-Louis, Center of Clinical Investigations in Biotherapies of Cancer CBT501, Université Paris Cité, F-75010 Paris, France.
  • Blanchoin L; Cytomorpholab, University Grenoble-Alpes, CEA, CNRS, INRA, Laboratoire de Phyiologie Cellulaire & Végétale, F-38054 Grenoble, France.
  • Théry M; Human Immunology, Pathophysiology, Immunotherapy, INSERM Unit 976, Institut de Recherche St Louis, AP-HP, Hôpital Saint-Louis, Université Paris Cité, F-75010 Paris, France.
  • Brunet S; Cytomorpholab, University Grenoble-Alpes, CEA, CNRS, INRA, Laboratoire de Phyiologie Cellulaire & Végétale, F-38054 Grenoble, France.
Development ; 151(17)2024 Sep 01.
Article em En | MEDLINE | ID: mdl-39136544
ABSTRACT
Hematopoietic stem and progenitor cells (HSPCs) give rise to all cell types of the hematopoietic system through various processes, including asymmetric divisions. However, the contribution of stromal cells of the hematopoietic niches in the control of HSPC asymmetric divisions remains unknown. Using polyacrylamide microwells as minimalist niches, we show that specific heterotypic interactions with osteoblast and endothelial cells promote asymmetric divisions of human HSPCs. Upon interaction, HSPCs polarize in interphase with the centrosome, the Golgi apparatus, and lysosomes positioned close to the site of contact. Subsequently, during mitosis, HSPCs orient their spindle perpendicular to the plane of contact. This division mode gives rise to siblings with unequal amounts of lysosomes and of the differentiation marker CD34. Such asymmetric inheritance generates heterogeneity in the progeny, which is likely to contribute to the plasticity of the early steps of hematopoiesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Hematopoéticas Limite: Humans Idioma: En Revista: Development Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Células-Tronco Hematopoéticas Limite: Humans Idioma: En Revista: Development Ano de publicação: 2024 Tipo de documento: Article