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The Association of MMP-2, MMP-9, and MMP-13 Gene Polymorphisms With Knee Osteoarthritis in the Greek Population.
Milaras, Christos; Lepetsos, Panagiotis; Pampanos, Andreas; Kenanidis, Eustathios; Macheras, George A; Mavrogianni, Despoina; Dafou, Dimitra; Potoupnis, Michael; Tsiridis, Eleftherios.
Afiliação
  • Milaras C; 4th Orthopedic Department, KAT Hospital, Athens, GRC.
  • Lepetsos P; 4th Orthopedic Department, KAT Hospital, Athens, GRC.
  • Pampanos A; Medical Genetics Department, "Alexandra" General Hospital, Athens, GRC.
  • Kenanidis E; Academic Orthopedic Department, Papageorgiou General Hospital, Aristotle University of Thessaloniki, School of Medicine, Thessaloniki, GRC.
  • Macheras GA; Center of Orthopedic and Regenerative Medicine - Center of Interdisciplinary Research and Innovation, Aristotle University of Thessaloniki, School of Medicine, Thessaloniki, GRC.
  • Mavrogianni D; 4th Orthopedic Department, KAT Hospital, Athens, GRC.
  • Dafou D; 1st Obstetrics and Gynecology Department, "Alexandra" General Hospital, National and Kapodistrian University of Athens, Medical School, Athens, GRC.
  • Potoupnis M; Biology Department, Aristotle University of Thessaloniki, Thessaloniki, GRC.
  • Tsiridis E; Center of Orthopedic and Regenerative Medicine - Center of Interdisciplinary Research and Innovation, Aristotle University of Thessaloniki, School of Medicine, Thessaloniki, GRC.
Cureus ; 16(7): e65379, 2024 Jul.
Article em En | MEDLINE | ID: mdl-39184681
ABSTRACT
Introduction Primary knee osteoarthritis (OA) is a multifactorial degenerative joint disorder characterized by articular cartilage degradation. Matrix metalloproteinases (MMPs) have been reported to play a vital role in OA pathogenesis, significantly contributing to extracellular matrix (ECM) catabolism. The purpose of this study is to investigate the association of MMP-2 -1575G/A (rs243866), MMP-9 836A/G (rs17576), and MMP-13 -77A/G (rs2252070) gene polymorphisms with knee OA in the Greek population. Methods One hundred patients (24% males, mean age 68.3 years) with primary knee OA were included in the study along with 100 controls (47% males, mean age 65.2 years). Genotypes were identified through polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) technique. Allelic and genotypic frequencies were compared between patients and controls. Results The MMP-13 -77A/G polymorphism was significantly associated with knee OA in the crude analysis (P = 0.008). After binary logistic regression analysis, the dominant model of the MMP-13-77A/G (AG + GG versus AA) was found to be associated with increased risk for knee OA (odds ratio (OR) = 2.290, 95% confidence interval (95%CI) = 1.059-4.949, P= 0.035). Compared to the A allele, the G allele in the MMP-13rs2252070 locus was a predictive factor for knee OA (OR = 2.351, 95%CI = 1.134-4.874, P= 0.022). No significant associations were detected for the MMP-2 -1575G/A and MMP-9 836A/G polymorphisms (P > 0.05). Conclusions The present study shows that the MMP-2 -1575G/A and MMP-9 836A/G polymorphisms are not significantly associated with primary knee OA in the Greek population. The MMP-13 -77A/G was found to be a significant risk factor for knee OA in the Greek population. Additional research is needed to verify this association in larger and different populations, in different joints, to elucidate the role of this single nucleotide polymorphism (SNP) in OA pathogenesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Cureus Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Cureus Ano de publicação: 2024 Tipo de documento: Article