Chromatographic Purification and Polishing of AAV Particles.
Methods Mol Biol
; 2848: 249-257, 2025.
Article
em En
| MEDLINE
| ID: mdl-39240527
ABSTRACT
The production of Adeno-associated virus (AAV) vectors in the lab setting has typically involved expression in adherent cells followed by purification through ultracentrifugation in density gradients. This production method is, however, not easily scalable, presents high levels of cellular impurities that co-purify with the virus, and results in a mixture of empty and full capsids. Here we describe a detailed AAV production protocol that overcomes these limitations through AAV expression in suspension cells followed by AAV affinity purification and AAV polishing to separate empty and full capsids, resulting in high yields of ultra-pure AAV that is highly enriched in full capsids.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Dependovirus
/
Vetores Genéticos
Limite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Ano de publicação:
2025
Tipo de documento:
Article