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RAP1-GTPase immunostaining is altered in human precancerous and cancerous cervical lesions.
de Vasconcelos, Paula Cristina; Freitas, Tulio Resende; de Araújo Lopes, Lara Verônica; Peixoto, Larissa Rodrigues; Xavier, Marcelo Pascoal; Cançado Figueiredo, Anna Carolina; Dias, Karolina Lopes; de Oliveira, Jaqueline Germano; de Oliveira Salles, Paulo Guilherme; Vago, Annamaria Ravaro; de Paula Sabino, Adriano; de Lima Rocha, Maria Gabrielle.
Afiliação
  • de Vasconcelos PC; Department of Clinical & Toxicological Analysis, College of Pharmacy, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
  • Freitas TR; Department of Clinical & Toxicological Analysis, College of Pharmacy, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
  • de Araújo Lopes LV; Department of Morphology, Institute of Biological Sciences, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
  • Peixoto LR; Department of Morphology, Institute of Biological Sciences, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
  • Xavier MP; Department of Pathological Anatomy, College of Medicine - Federal University of Minas Gerais, Belo Horizonte, Minas Gerais, 30130-100, Brazil.
  • Cançado Figueiredo AC; Integrated Research Group on Biomarkers, Renée Rachou Research Institute - FIOCRUZ, Belo Horizonte, Minas Gerais, 30190-003, Brazil.
  • Dias KL; Laboratory of Cellular & Molecular Immunology, Renée Rachou Research Institute - FIOCRUZ, Belo Horizonte, Minas Gerais, 30190-003, Brazil.
  • de Oliveira JG; Laboratory of Cellular & Molecular Immunology, Renée Rachou Research Institute - FIOCRUZ, Belo Horizonte, Minas Gerais, 30190-003, Brazil.
  • de Oliveira Salles PG; Laboratory of Pathological Anatomy - Luxemburgo Hospital, Belo Horizonte, Minas Gerais, 30380-490, Brazil.
  • Vago AR; Department of Morphology, Institute of Biological Sciences, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
  • de Paula Sabino A; Department of Clinical & Toxicological Analysis, College of Pharmacy, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
  • de Lima Rocha MG; Department of Clinical & Toxicological Analysis, College of Pharmacy, Belo Horizonte, Minas Gerais, 31270-901, Brazil.
Biomark Med ; 18(17-18): 771-785, 2024.
Article em En | MEDLINE | ID: mdl-39254347
ABSTRACT

Aim:

This study investigated RAP1 immunostaining variation in different cell types during CC progression.

Methods:

Paraffin-embedded cervical tissues from 101 patients were categorized into control, pre-neoplastic and neoplastic groups. RAP1 immunolocalization, HPV detection and genotyping were performed. A semiquantitative immunoreactive score was employed to compare labeling intensity, cellular localization, nuclear labeling, percentage and distribution of reactive cells.

Results:

73% (72/99) of cervical specimens were HPV+. RAP1 was localized in the nucleus and cytoplasm of all samples. Cytoplasmic RAP1 immunoscore was higher than nuclear score in all CC groups. RAP1 intensity increased with lesion severity. SCC samples exhibited predominantly intense RAP1 immunostaining.

Conclusion:

RAP1 is an efficient biomarker for detecting invasive CC lesions but has limited utility in distinguishing SCC grades.
[Box see text].
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lesões Pré-Cancerosas / Neoplasias do Colo do Útero Limite: Adult / Aged / Female / Humans / Middle aged Idioma: En Revista: Biomark Med Ano de publicação: 2024 Tipo de documento: Article
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lesões Pré-Cancerosas / Neoplasias do Colo do Útero Limite: Adult / Aged / Female / Humans / Middle aged Idioma: En Revista: Biomark Med Ano de publicação: 2024 Tipo de documento: Article