HASTY-mediated miRNA dynamics modulate nitrogen starvation-induced leaf senescence in Arabidopsis.
Nat Commun
; 15(1): 7913, 2024 Sep 10.
Article
em En
| MEDLINE
| ID: mdl-39256370
ABSTRACT
Nitrogen (N) deficiency responses are essential for plant survival and reproduction. Here, via an expression genome-wide association study (eGWAS), we reveal a mechanism that regulates microRNA (miRNA) dynamics necessary for N deficiency responses in Arabidopsis. Differential expression levels of three NAC transcription factor (TF) genes involved in leaf N deficiency responses among Arabidopsis accessions are most significantly associated with polymorphisms in HASTY (HST), which encodes an importin/exportin family protein responsible for the generation of mature miRNAs. HST acts as a negative regulator of N deficiency-induced leaf senescence, and the disruption and overexpression of HST differently modifies miRNA dynamics in response to N deficiency, altering levels of miRNAs targeting transcripts. Interestingly, N deficiency prevents the interaction of HST with HST-interacting proteins, DCL1 and RAN1, and some miRNAs. This suggests that HST-mediated regulation of miRNA dynamics collectively controls regulations mediated by multiple N deficiency response-associated NAC TFs, thereby being central to the N deficiency response network.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
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Arabidopsis
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Folhas de Planta
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Regulação da Expressão Gênica de Plantas
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Proteínas de Arabidopsis
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MicroRNAs
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Nitrogênio
Idioma:
En
Revista:
Nat Commun
Ano de publicação:
2024
Tipo de documento:
Article