Whole-cell Rieske non-heme iron biocatalysts.
Methods Enzymol
; 703: 243-262, 2024.
Article
em En
| MEDLINE
| ID: mdl-39260998
ABSTRACT
Rieske non-heme iron oxygenases (ROs) possess the ability to catalyze a wide range of reactions. Their ability to degrade aromatic compounds is a unique characteristic and makes ROs interesting for a variety of potential applications. However, purified ROs can be challenging to work with due to low stability and long, complex electron transport chains. Whole cell biocatalysis represents a quick and reliable method for characterizing the activity of ROs and harnessing their metabolic potential. In this protocol, we outline a step-by-step protocol for the overexpression of ROs for whole cell biocatalysis and characterization. We have utilized a caffeine-degrading, N-demethylation system, expressing the RO genes ndmA and ndmD, as an example of this method.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Biocatálise
Idioma:
En
Revista:
Methods Enzymol
Ano de publicação:
2024
Tipo de documento:
Article