Activation of fibroblasts by plasma cells via PDGF/PDGFR signaling in IgG4-related sialadenitis.
J Clin Exp Hematop
; 64(3): 223-231, 2024.
Article
em En
| MEDLINE
| ID: mdl-39343610
ABSTRACT
IgG4-related sialadenitis (IgG4-SA) is one of the IgG4-related disease. The histological features of IgG4-SA include dense lymphoplasmacytic infiltrates and fibrosis. This study aimed to reveal the involvement of plasma cells in the development of fibrosis and the mechanism underlying fibrosis in IgG4-SA. Hematoxylin-eosin staining, Azan staining, silver staining, and immunohistochemistry (IHC) were performed on IgG4-SA and chronic sialadenitis specimens, and theses samples were analyzed by image analysis software. Histological spatial analysis was used to analyze the localization of IHC-positive cells and the distances between these cells. In the IgG4-SA group, many secondary lymphoid follicles with germinal centers were found, and many collagen fibers developed around these germinal centers. Collagen fibers composed mainly of type I collagen was abundant at sites away from secondary lymphoid follicles, and reticular fibers composed of type III collagen was abundant near secondary lymphoid follicles. Many FAP+ fibroblasts and MUM1+ plasma cells were localized near secondary lymphoid follicles. Histological spatial analysis demonstrated that 90.4% of MUM1+ plasma cells accumulated within 20 µm of FAP+ fibroblasts. Multiple immunofluorescence assays revealed that MUM1+ plasma cells expressed platelet-derived growth factor (PDGF) ß, and FAP+ fibroblasts expressed PDGF receptor (PDGFR) ß and pSTAT3 in IgG4-SA. We have shown that fibrosis is localized around secondary lymphoid follicles and that fibroblasts are activated by plasma cells via PDGF/PDGFR signaling in IgG4-SA.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Plasmócitos
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Sialadenite
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Imunoglobulina G
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Transdução de Sinais
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Fibroblastos
Limite:
Female
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Humans
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Male
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Middle aged
Idioma:
En
Revista:
J Clin Exp Hematop
Ano de publicação:
2024
Tipo de documento:
Article