Conformational requirements for activity of salmon calcitonin.
Endocrinology
; 117(3): 801-5, 1985 Sep.
Article
em En
| MEDLINE
| ID: mdl-4017965
ABSTRACT
A series of deletion-substitution analogs of salmon calcitonin (SCT) have been prepared containing combinations of a glycine substitution in position 8 and deletions of serine-2 and tyrosine-22. Biological activity of the analogs with respect to native SCT were determined in the rat hypocalcemic assay and by studying stimulation of cAMP formation and competition for binding of 125I-labeled SCT in T47 D human breast cancer cells. It was found that each of the analogs retained full potency, irrespective of the means of assessment. It is suggested that conservation of the alpha-helical region of SCT, along with the overall tertiary structure, are more important for peptide potency than chain length per se.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Calcitonina
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Revista:
Endocrinology
Ano de publicação:
1985
Tipo de documento:
Article