Analysis of the expression of cloned HLA class I genes in mouse transfected L cells by quantitative flow cytometry.
Cytometry
; 6(5): 414-21, 1985 Sep.
Article
em En
| MEDLINE
| ID: mdl-4042783
ABSTRACT
By using a calibrated cell sorter and monoclonal antibodies conjugated to fluorochromes, a quantitative analysis of the levels of expression of HLA class I molecules at the surface of cloned murine L cells transfected with purified A3, B7, or CW3 genes was performed and compared with radioimmunoassay data. We selected clones of heterogeneous levels of HLA class I expression, which were shown to remain constant over a period of 4 mo in absence of HAT selection and not to be correlated to the DNA copy number of the corresponding integrated gene.
Buscar no Google
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Citometria de Fluxo
/
Antígenos HLA
Limite:
Animals
/
Humans
Idioma:
En
Revista:
Cytometry
Ano de publicação:
1985
Tipo de documento:
Article